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Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Article DOI: 10.3791/50087-v • 13:55 min • February 3rd, 2013
February 3rd, 2013 •
1, 1,2,3,4, 1
1Department of Pathology, New York University School of Medicine, 2New York University Center for Health Informatics and Bioinformatics, 3NYU Cancer Institute, 4Department of Pathology and Yale Cancer Center, Yale University School of Medicine

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Summary
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Here we describe a protocol for simultaneous detection of histone modifications by immunofluorescence and DNA sequences by DNA FISH followed by 3D microscopy and analyses (3D immuno-DNA FISH).

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Immunofluorescence DNA FISH 3D-preserved Interphase Nuclei 3D Nuclear Organization Fluorescent In Situ Hybridization DNA Probes 3D Confocal Microscopy Gene Loci Chromosomal Sub-regions Nuclear Architecture Genomic Loci Nuclear Proteins Histones Transcription Machinery Nuclear Sub-compartments Epitope Preservation Chromatin Modifications
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