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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
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Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

Article DOI: 10.3791/50112
January 2nd, 2013 •
1, 1, 1
1The Walter and Eliza Hall Institute of Medical Research

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January 2nd, 2013

A method for isolation of adherent inflammatory leukocytes from brain blood vessels of Plasmodium berghei ANKA-infected mice is described. The method allows quantification as well as phenotypic characterization of isolated leukocytes after staining with fluorescent antibodies and subsequent analysis by flow cytometry.

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