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Immunology and Infection
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Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

DOI:
10.3791/50112-v

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12:48 min

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January 02, 2013

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, ,
1The Walter and Eliza Hall Institute of Medical Research

Chapters

  • 00:05Title
  • 01:52Infection of Mice with P. berghei-ANKA
  • 06:49Isolation of Brain-sequestered Leukocytes (BSL)
  • 09:10Immunophenotyping of BSL by Multicolor Flow Cytometry
  • 10:27Representative Analysis of Isolated BSL Populations
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

A method for isolation of adherent inflammatory leukocytes from brain blood vessels of Plasmodium berghei ANKA-infected mice is described. The method allows quantification as well as phenotypic characterization of isolated leukocytes after staining with fluorescent antibodies and subsequent analysis by flow cytometry.

Tags

Keywords: Brain-sequestered LeukocytesPlasmodium Berghei ANKAExperimental Cerebral MalariaIntracardial PerfusionPercoll GradientFlow CytometryInflammatory LeukocytesParasitic Infection

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