Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice

The overall goal of this procedure is to isolate adherent inflammatory leukocytes from brain blood vessels of plasmodium burge eye anca infected mice to induce experimental cerebral malaria. Mice are infected with plasmodium berge eye anca parasitized red blood cells by injection. After the mice begins showing signs of disease, the circulating cells of malaria infected mice are removed by intraoral perfusion.

The brains are then harvested and the tissue is digested with enzymes to allow isolation of inflammatory leukocytes. Multicolor flow cytometry is then performed to immuno phenotype. The brain sequestered leukocytes analysis of the resulting data reveals the percentage and absolute numbers of leukocytes migrating to the brain of malaria infected mice.