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JoVE Journal
Immunology and Infection

This content is Open Access.

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
 

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

Article doi: 10.3791/50116
June 12th, 2013

Summary June 12th, 2013

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

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