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Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue

DOI:
10.3791/50126-v

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11:31 min

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February 11, 2013

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, , , ,
1Weldon School of Biomedical Engineering,Purdue University, 2Department of Biological Sciences,Purdue University

Chapters

  • 00:05Title
  • 02:12Brain Removal with In situ Devices
  • 05:17Tissue Processing and Clearing
  • 07:05Panorama Imaging of Full Tissue and Device Interface
  • 09:26Results: Intact Histological Characterization of Brain-implanted Microdevices and Surrounding Tissue
  • 10:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a histological method for capturing, labeling, optically clearing, and imaging the intact brain tissue interface around chronically implanted microdevices in rodent brain tissue. Results from the techniques comprising this method are useful for understanding the impact of various penetrating brain-implants on their surrounding tissue.

Tags

Brain-implanted MicrodevicesMicroelectrode ArraysGlial ScarHistological AnalysisRodent BrainFluorescent Antibody LabelingOptical ClearingBiological Interface

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