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Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
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JoVE Journal Neuroscience
Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

DOI:
10.3791/50150-v

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10:33 min

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March 04, 2013

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, , , ,
1Institute of Genetics,University of Mainz, 2Department of Anatomy and Neuroscience,University of Melbourne

Chapters

  • 00:05Title
  • 01:14Embryo Collection and Preparation
  • 04:21Dye Injection of Individual Neruons
  • 08:26DiI Labeled Neurons
  • 10:01Conclusion

Summary

Automatic Translation

Automatic Translation

We present a technique for labeling single neurons in the central nervous system (CNS) of Drosophila embryos, which allows the analysis of neuronal morphology by either transmitted light or confocal microscopy.

Tags

Keywords: Single Cell LabelingDrosophila MelanogasterCentral Nervous SystemDiIJuxtacellular InjectionNeuronal Cell MorphologyPhotoconversionConfocal MicroscopyGenetically Encoded Fluorescent MarkersMutant Phenotypes

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