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Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique
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Biology
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JoVE Journal Biology
Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

Evaluation of Respiratory System Mechanics in Mice using the Forced Oscillation Technique

DOI:
10.3791/50172-v

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13:10 min

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May 15, 2013

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, , , ,
1Meakins-Christie Laboratories, Department of Medicine,McGill University, 2SCIREQ Scientific Respiratory Equipment Inc.

Chapters

  • 00:05Title
  • 01:41Preparing the flexiVent FX System
  • 03:19Tracheotomy and Cannulation
  • 04:55Mechanical Ventilation
  • 06:26Lung Function Measurements
  • 10:06Typical Respiratory Measurements and those Following a Methacholine Challenge
  • 12:38Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol provides a detailed step-by-step description of the procedures required to execute measurements of respiratory system mechanics as well as the assessment of airway responsiveness to inhaled methacholine in mice using the forced oscillation technique (flexiVent; SCIREQ Inc, Montreal, Qc, Canada).

Tags

Keywords: Respiratory System MechanicsForced Oscillation TechniqueLung FunctionAirway ResponsivenessMethacholineMiceAnimal ModelFlexiVentRespiratory MechanicsAirway PathologyOxidative Stress

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