Mouse Lung Function
Mouse Lung Function - Related Videos
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
04:21
Protein Transfection of Mouse Lung
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Cited by 1 •
2013
Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.
07:45
Modeling Dysplastic and Functional Lung Alveolar Repair after Influenza Infection
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2025
This protocol provides instructions for intranasal administration of IAV and downstream analysis of mouse lung damage and associated transcriptional changes to lung cell types.
06:57
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Cited by 48 •
2015
The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.
FAQs
Breathing depends on pressure differences within the respiratory system. These differences move air through the conducting airways and into the alveoli during inhalation, then support outward airflow during exhalation. Measuring respiratory pressure together with flow helps researchers evaluate how effectively the mouse generates and maintains the pressure changes required for ventilation.
The alveolar-capillary barrier separates air in the alveoli from the blood while permitting oxygen and carbon dioxide to cross. Its performance therefore influences gas exchange, even when airflow measurements alone may appear informative. Assessing lung physiology alongside gas-exchange consequences helps investigators identify whether an experimental condition affects ventilation, exchange across this barrier, or both.
Airway resistance describes opposition to airflow, whereas lung compliance reflects how readily the lungs change volume in response to pressure. Changes in either property can alter measured flow, pressure, or tidal volume, but they represent different physiological features. Considering both prevents researchers from attributing every change in breathing measurements to the same underlying process.
A controlled challenge provides a standardized condition for examining how the respiratory system responds to a defined stimulus. Comparing flow, pressure, tidal volume, or related measurements before and during the challenge can expose functional changes that may not be evident at baseline. This approach is useful for characterizing altered pulmonary responses in experimental models.
Core measurements include respiratory flow, pressure, and tidal volume, with assessments also addressing airway resistance and lung compliance. Together, these variables describe air movement, the pressure conditions supporting breathing, the amount of air moved per breath, and mechanical properties of the lungs. Their combined interpretation gives a broader view than any single measurement.
Investigators measure these functions to characterize normal pulmonary physiology and to detect changes associated with inflammation, infection, genetic mutations, environmental exposures, or experimental treatments. The resulting data can help distinguish altered respiratory performance from normal variation and support evaluation of potential therapies. Mouse studies therefore connect physiological measurements with disease modeling and treatment research.