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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Article DOI: 10.3791/50193-v • 10:11 min • March 7th, 2013
March 7th, 2013 •
1, 1
1Division of Hematology-oncology, Department of Medicine, Medical University of South Carolina

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Determining donor cell engraftment presents a challenge in mouse bone marrow transplant models that lack well-defined phenotypical markers. We described a methodology to quantify male donor cell engraftment in female transplant recipient mice. This method can be used in all mouse strains for the study of HSC functions.

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Quantitative Real-time PCR Donor Cell Engraftment Murine Bone Marrow Transplantation Model Hematopoietic Stem Cells Engraftment Measurement Flow Cytometry Cell Surface Markers Genetically Modified Mouse Strains PCR-based Technique Transplant Recipient Mice Peripheral Blood Samples Bone Marrow Samples Genomic DNA Isolation
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