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巨脂质体制剂成像和膜片钳电
JoVE Journal
Biology
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JoVE Journal
Biology
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
Please note that all translations are automatically generated.
Click here for the English version.
巨脂质体制剂成像和膜片钳电
DOI:
10.3791/50227-v
•
09:03 min
•
June 21, 2013
•
Marcus D. Collins
,
Sharona E. Gordon
1
Department of Physiology and Biophysics
,
University of Washington
Chapters
00:05
Title
01:19
Deposition of Lipids from Organic Solvents: Classical Protocol
03:13
Electroformation of Giant Liposomes
05:19
Deposition of Lipids by Controlled Dehydration of Small Liposomes
07:10
Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
08:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
重构功能膜蛋白组成界定成巨脂质体是一个功能强大的方法,结合膜片钳电。然而,传统的巨头脂质体产量可能与蛋白质的稳定性不相容。我们描述生产从单纯的脂质的巨脂质体或小脂质体含有离子通道的协议。
Tags
Giant Liposomes
Ion Channels
Electrophysiology
Patch-clamp
Electroformation
Lipid Membranes
Reconstitution
Small Liposomes
Dehydration
Vapor Equilibrium
Saturated Salt Solutions
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