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Inleiding tot de vaste Ondersteunde Membrane Gebaseerd Electrofysiologie
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Introduction to Solid Supported Membrane Based Electrophysiology
DOI:

19:56 min

May 11, 2013

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Chapters

  • 00:01Title
  • 00:06Introduction
  • 01:25Background: Measuring principles
  • 02:51Background: The SSM Setup
  • 05:25Experimental procedure: Mounting the cuvette
  • 08:34Experimental procedure: Checking membrane parameters
  • 09:47Experimental procedure: Adding the protein sample
  • 10:53Experimental procedure: Defining the flow protocol
  • 11:48Experimental procedure: Measuring transporter signals
  • 14:12Data Analysis: Reconstruction of the transporter current
  • 15:07Data Analysis: Representative results
  • 17:26Data Analysis: Artifacts & control measurements
  • 19:00Conclusion

Summary

Automatic Translation

Hier presenteren we een elektrofysiologische methode gebaseerd op vaste drager membranen met de nadruk op de toepassing daarvan voor de karakterisering van elektrogene membraan transporters.

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