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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
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Biology
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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

DOI:
10.3791/50235-v

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10:53 min

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July 03, 2013

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, ,
1Institute of Pharmacology,University of Duisburg-Essen , 2Division of Experimental Cardiology,University of Heidelberg

Chapters

  • 00:05Title
  • 01:26Cleaning the Tissue
  • 03:11Enzymatic Digestions and Precipitation
  • 06:33Loading of Myocytes with Fluo-3AM
  • 07:53Representative Cardiomyocytes and Calcium Transient Recordings
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the isolation of human atrial myocytes which can be used for intracellular Ca2+ measurements in combination with electrophysiological patch-clamp studies.

Tags

Keywords: Human Atrial MyocytesPatch-clampCa2+ TransientsMembrane CurrentsAtrial FibrillationCell IsolationCollagenaseProteaseCa2+ Buffering

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