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端粒长度和端粒酶活性;阴阳细胞衰老
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Biology
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JoVE Journal Biology
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
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端粒长度和端粒酶活性;阴阳细胞衰老

DOI:
10.3791/50246-v

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12:08 min

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May 22, 2013

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, ,
1Department of Medicine,Albert Einstein College of Medicine , 2Diabetes Research and Training Center,Albert Einstein College of Medicine , 3Department of Genetics,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 01:49Telomere Length Measurement using Quantitative Real-Time PCR
  • 05:50Quantitative Telomerase Detection Activity Measurement
  • 09:47Results: Quantitative Real-Time PCR is Used to Determine Telomere length and Telomerase Activity in Human Leukocytes
  • 11:41Conclusion

Summary

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Automatic Translation

描述一个准确的，短的，复杂的和廉价的方法，使用定量RT-PCR在多个组织和物种评估端粒长度。此外，我们将介绍一个简单的检测，以评估作为一个互补的骨干测试端粒长度的端粒酶活性。

Tags

Telomere LengthTelomerase ActivityCell SenescenceChromosome AttritionCell DivisionCell AgingQRT-PCRTelomere SequenceTelomere AssayTelomerase Assay

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