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Neuroscience

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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
 

Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures

Article doi: 10.3791/50273
April 3rd, 2013

Summary April 3rd, 2013

The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in the CNS to study and characterize synaptic proteins in rat hippocampal CA1 pyramidal neurons.

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