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JoVE Journal Biology
Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
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Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

Article DOI: 10.3791/50289
September 24th, 2013 •
1, 1,2, 1, 1, *1, *1
1Cardiovascular Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Division of Cardiology, Sapienza University
* These authors contributed equally

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September 24th, 2013

Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.

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