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Biology

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Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes
 

Isolation, Culture, and Functional Characterization of Adult Mouse Cardiomyoctyes

Article doi: 10.3791/50289
September 24th, 2013

Summary September 24th, 2013

Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.

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