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Chapters
- 00:05Title
- 01:33Cardiomyocyte Isolation
- 07:32Operating the MMSYS System
- 10:43Cardiomyocyte Morphology and MMSYS System Analysis
- 12:28Conclusion
Here we describe the isolation of adult mouse cardiomyoctyes using a Langendorff perfusion system. The resulting cells are Ca2+-tolerant, electrically quiescent and can be cultured and transfected with adeno- or lentiviruses to manipulate gene expression. Their functionality can also be analyzed using the MMSYS system and patch clamp techniques.
