Erratum: Generation of Transgenic C. elegans by Biolistic Transformation

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December 12th, 2012

In This Article

Summary

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.

Abstract

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.

Protocol

The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect. The volume has been corrected from:

The spermidine is unstable in aqueous solution. We make 70μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.

to:

The spermidine is unstable in aqueous solution. We make 16μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.

Disclosures

No conflicts of interest declared.

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Tags

C Elegans GeneticsGenetic TransformationParticle BombardmentTransgenic OrganismsGene DeliveryModel OrganismGenetic EngineeringDNA Introduction