The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.
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December 12th, 2012
The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.
The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect.
The volume for a solution in, Generation of Transgenic C. elegans by Biolistic Transformation, was incorrect. The volume has been corrected from:
The spermidine is unstable in aqueous solution. We make 70μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.
to:
The spermidine is unstable in aqueous solution. We make 16μl spermidine aliquots and store at -20°C. We then add 1mL of water right before use to obtain a 0.1M solution. This solution must be prepared fresh for each bombardment.
No conflicts of interest declared.