Exosome Mirna Profiling
Exosome Mirna Profiling - Related Videos
06:59
Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
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Cited by 17 •
2016
Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.
10:45
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
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Cited by 45 •
2013
The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.
08:49
Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
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Cited by 59 •
2016
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.
03:59
miRNA Extraction: A Method to Extract miRNA from Plasma Sample
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2023
MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, and could represent ideal candidates for early detection of lung cancer. In the featured protocol, we will isolate microRNA from patient blood samples for early lung cancer screening.
09:43
Isolation and Characterization of RNA-Containing Exosomes
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Cited by 515 •
2012
This paper demonstrates methods for the isolation, purification and detection of exosomes, as well as techniques for analysis of their molecular content. These methods are adaptable for exosome isolation from both cell culture media and biological fluids, and can beyond analysis of molecular content also be useful in functional studies.
FAQs
The profiles provide a molecular view of the miRNAs enclosed in exosomes and allow researchers to compare cargo between samples. Differences in miRNA abundance can be examined as evidence of disease-associated molecular changes or as clues to how extracellular vesicles participate in communication with recipient cells. This makes profiling useful for linking vesicle cargo with cellular activity.
Membrane disruption releases the RNA enclosed within isolated exosomes, making it accessible for extraction and downstream measurement. Without this step, the analysis would not directly assess the vesicle-contained miRNAs described by the sample. The extracted RNA can then be evaluated with reverse-transcription quantitative PCR, microarrays, or next-generation sequencing to determine miRNA abundance.
These approaches are alternative measurement platforms used after RNA extraction. Reverse-transcription quantitative PCR, microarrays, and next-generation sequencing each support assessment of miRNA abundance, allowing profiles from different samples to be compared. The overview does not assign one platform as universally preferable; their shared purpose is to characterize molecular differences in exosome-associated miRNAs.
Biofluids and cell cultures are the two sample settings identified for this analysis. Exosomes are isolated from either source before their membranes are disrupted and the enclosed RNA is extracted. Using these distinct biological materials lets investigators examine exosome-associated miRNA patterns in different experimental contexts within biology research.
Comparing miRNA profiles across samples can reveal differences in exosome-associated miRNA abundance. When those differences reflect disease-associated molecular changes, the measured miRNAs may be investigated as potential biomarkers. The method therefore helps researchers connect molecular patterns with biological or disease-related states, making it useful for exploratory biomarker studies in biology.
Applications extend across several biological questions rather than one disease model. The approach supports studies of development, cancer, immune responses, and therapeutic communication. In each setting, researchers can examine exosome-associated miRNA patterns as molecular information related to cellular activity, intercellular signaling, or communication with recipient cells.
Because exosomes participate in communication with recipient cells, their miRNA contents provide a way to examine one molecular component of that process. Profiling can show which miRNAs are present and how their abundance differs between samples, helping researchers investigate how extracellular vesicles may influence recipient-cell biology and clarify therapeutic communication.