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Biology

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Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)
 

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

Article doi: 10.3791/50317
May 7th, 2013

Summary May 7th, 2013

Targeted-esterase induced dye loading (TED) supports the analysis of intracellular calcium store dynamics by fluorescence imaging. The method bases on targeting of a recombinant Carboxylesterase to the endoplasmic reticulum (ER), where it improves the local unmasking of synthetic low-affinity Ca2+ indicator dyes in the ER lumen.

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