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In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
 

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

Article DOI: 10.3791/50322
February 22nd, 2013

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Summary February 22nd, 2013

The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.

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