Pulmonary Microvasculature
Pulmonary Microvasculature - Related Videos
02:58
Two-Photon Microscopy for In Vivo Imaging of Mouse Brain Microvasculature
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2025
Source: Nishimura, C. et al. Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation. J. Vis. Exp. (2016). This video demonstrates the in vivo visualization of cortical capillaries in the mouse brain using two-photon microscopy. It outlines the steps for injecting a fluorescent dye into an anesthetized mouse with a thin-skull cortical window, preparing the setup for imaging, and acquiring detailed images of brain...
08:55
In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer
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Cited by 17 •
2013
The endothelial glycocalyx/endothelial surface layer is ideally studied using intravital microscopy. Intravital microscopy is technically challenging in a moving organ such as the lung. We demonstrate how simultaneous brightfield and fluorescent microscopy may be used to estimate endothelial surface layer thickness in a freely-moving in vivo mouse lung.
08:17
Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery
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Cited by 8 •
2017
The following protocol describes the methodology for the acquisition and analysis of echocardiographic images used to obtain the Left Atrial Volume (LAV), Aorta (Ao) diameter, and Pulmonary Artery (PA) diameter in mice. This technique is a non-invasive, non-terminal procedure that allows assessment of the cardiopulmonary function.
09:39
Spatial Temporal Analysis of Fieldwise Flow in Microvasculature
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Cited by 7 •
2019
To quantify microvascular flow from high speed capillary flow image sequences, we developed STAFF (Spatial Temporal Analysis of Fieldwise Flow) software. Across the full image field and over time, STAFF evaluates flow velocities and generates a sequence of color-coded spatial maps for visualization and tabular output for quantitative analyses.
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
FAQs
Vessel diameter and pressure help determine how blood is distributed through the exchange region. Narrowing or abnormal pressure can disturb the balance required for effective oxygen uptake and carbon dioxide removal, while appropriate dimensions and pressures support close blood-to-air contact. These variables therefore link pulmonary circulation with respiratory performance.
Endothelial function matters because the vessel lining helps maintain the conditions needed for effective exchange. When endothelial behavior is impaired, regulation of the microvascular network may become abnormal, contributing to altered circulation and tissue fluid accumulation. This makes endothelial status relevant when investigating pulmonary hypertension, edema, and acute lung injury.
Vascular remodeling refers to structural changes in small vessels that can alter their dimensions and pressure environment around alveoli. These changes may disrupt exchange and contribute to pulmonary hypertension. Studying remodeling helps connect microscopic vascular alterations with clinically important changes in pulmonary circulation and function.
Examining the pulmonary microvasculature connects vessel-level changes with broader physiological outcomes, including gas exchange and pulmonary circulation. It also helps researchers investigate how vascular remodeling or endothelial dysfunction relates to pulmonary hypertension, edema, and acute lung injury. This information supports medical diagnosis, treatment, and research focused on vascular causes of respiratory impairment.
These conditions can involve disruption of the delicate vascular environment surrounding the alveoli. Altered endothelial function or abnormal pressure may disturb fluid balance and reduce the efficiency of gas exchange. Evaluating the microvasculature therefore helps clinicians and researchers relate local vascular dysfunction to the broader respiratory consequences observed in edema and acute lung injury.
Research in this area examines how pulmonary blood flow and alveolar gas exchange depend on one another. It provides a framework for relating cardiovascular changes, such as abnormal pulmonary pressures, to respiratory outcomes involving oxygen and carbon dioxide movement. This connection is especially useful when studying pulmonary hypertension, vascular remodeling, and other disorders affecting pulmonary circulation.