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JoVE Journal
Immunology and Infection

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An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
 

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Article DOI: 10.3791/50332
June 12th, 2013

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Summary June 12th, 2013

Monocyte-derived macrophages are important cells of the innate immune system. Here, we describe an easy to use in vitro model to generate these cells. Using gradient centrifugation, negative bead isolation and specific cell culture conditions, monocyte-derived macrophages can be generated for phenotypic and functional studies.

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