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09:17 min
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April 24, 2013
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Summary
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Hier beschrijven we twee testen die zijn opgericht om leeftijdsafhankelijke neurodegeneratie van de dopaminerge (DA) neuronen studeren in<em> Drosophila</em>: Het klimmen / schrikken geïnduceerde negatieve geotaxis assay waarmee de functionele effecten van DA neuronen degeneratie en tyrosine hydroxylase immunokleuring die wordt gebruikt om DA neuronen in volledige hersenen mounts identificeren en tellen bestuderen.
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Cite this Article
Barone, M. C., Bohmann, D. Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining. J. Vis. Exp. (74), e50339, doi:10.3791/50339 (2013).
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