19,015 Views
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09:17 min
April 24, 2013
DOI:
10.3791/50339-v
ここでは、ドーパミン作動性に(DA)ニューロンの年齢依存性神経変性を研究するために設立されている2つのアッセイを記述する<em>ショウジョウバエ</em>:登山/ DAニューロンの変性と脳全体のマウントのDAニューロンを特定し、カウントするために使用されているチロシン水酸化酵素の免疫の機能的効果を研究することができます驚愕誘発性の負の走地性アッセイ。
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Barone, M. C., Bohmann, D. Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining. J. Vis. Exp. (74), e50339, doi:10.3791/50339 (2013).
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