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A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
JoVE Journal
Medicine
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JoVE Journal Medicine
A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics

DOI:
10.3791/50363-v

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12:09 min

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June 16, 2013

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, , , , ,
1Brain Tumor Research Centre,Hospital for Sick Children, Toronto Medical Discovery Tower, 2Ontario Cancer Institute,Princess Margaret Hospital, 3Neurosurgery,Toronto Western Hospital

Chapters

  • 00:05Title
  • 02:14Bone Marrow Reconstitution
  • 03:34Intracranial Window Generation
  • 06:50Stereotactic Radiation
  • 08:39In vivo Two Photon Laser Microscopy
  • 09:53Results: Potential Problems Encountered with this Novel High-resolution In vivo Imaging Technique and the Potential Uses of the Model
  • 11:25Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a novel in vivo imaging technique that couples fluorescent chimeric mice with intracranial windows and high-resolution 2-photon microscopy. This imaging platform aids studies of dynamic changes in brain tissue and microvasculature, at a single-cell level, following pathological insults and is adaptable to assess intracranial drug delivery and distribution.

Tags

Keywords: Intracranial WindowIntravital Imaging2-photon Confocal MicroscopyFluorescent Chimeric MiceGFP+ Bone MarrowMCherry GliomaCD31DextranSmall Animal Micro-irradiatorIonizing Irradiation

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