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Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
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Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

DOI:
10.3791/50364-v

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10:50 min

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June 29, 2013

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, , , , , ,
1The Texas Heart Institute at St. Luke’s Episcopal Hospital, 2Department of Vascular Surgery, The Ninth People’s Hospital, School of Medicine,Shanghai Jiao Tong University

Chapters

  • 00:05Title
  • 02:47Priming the Osmotic Pump
  • 03:58Creating Unilateral Hindlimb Ischemia in the Mouse
  • 06:30Imaging Specimen Preparation
  • 09:40Results: Representative Perfusion of the Vasculature in the Murine Hindlimb Ischemia Model
  • 10:27Conclusion

Summary

Automatic Translation

Automatic Translation

We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.

Tags

Keywords: Osmotic Drug DeliveryIschemic HindlimbMicrofil PerfusionMicro-computed TomographyPeripheral Arterial DiseasePreclinical ResearchAnimal ModelVascular BiologyAngiogenesisVascular Imaging

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