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A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
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A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Article DOI: 10.3791/50369 • 10:13 min • July 3rd, 2013
July 3rd, 2013 •
1, 1
1Department of Cell Biology, UT Southwestern Medical Center

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Summary
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Achieving a systems level understanding of cellular processes is a goal of modern-day cell biology. We describe here strategies for multiplexing luciferase reporters of various cellular function end-points to interrogate gene function using genome-scale RNAi libraries.

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Multiplexed Luciferase-based Screening Platform Cancer-associated Signal Transduction Cultured Cells Genome-scale Interrogation RNA Interference (RNAi) Gene Function Chemically Tractable Cancer Cell Vulnerabilities Mechanistic Basis Phenotypic Outcomes Molecularly Targeted Therapeutic Strategies Cellular Phenotypes RNAi-mediated Gene Targeting Multiplexed Reporter Systems High-content Screening Methodology Large Molecular Libraries
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