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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
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Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

DOI:
10.3791/50371-v

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08:05 min

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June 07, 2013

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, , , ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich

Chapters

  • 00:05Title
  • 01:09Material Preparation
  • 02:24Seed Dissection and Embryo Extraction
  • 03:49Embryo Isolation
  • 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.

Tags

Keywords: Arabidopsis ThalianaEarly-stage EmbryosIsolationEndospermSeed IntegumentsSeed CoatManual DissectionGlass MicrocapillaryRT-PCRRNA SequencingDNA MethylationFISHImmunostainingReporter Gene Assays

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