Journal
/
Biology
/
Effektiv og hurtig Isolering af Debuterende embryoner fra<em> Arabidopsis thaliana</em> Frø
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Effektiv og hurtig Isolering af Debuterende embryoner fra<em> Arabidopsis thaliana</em> Frø

DOI:
10.3791/50371-v

•

08:05 min

•

June 07, 2013

•

, , , ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich

Chapters

  • 00:05Title
  • 01:09Material Preparation
  • 02:24Seed Dissection and Embryo Extraction
  • 03:49Embryo Isolation
  • 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Vi rapporterer en effektiv og enkel metode til at isolere embryoner i de tidlige stadier af udvikling fra<em> Arabidopsis thaliana</em> Frø. Op til 40 embryoner kan isoleres i 1 time til 4 timer, afhængigt af den efterfølgende anvendelse. Proceduren er egnet til transkriptom, DNA-methylering, reporter-genekspression, immunfarvning og fluorescens<em> In situ</em> Hybridiserings analyser.

Tags

Arabidopsis ThalianaEarly-stage EmbryosIsolationEndospermSeed IntegumentsSeed CoatManual DissectionGlass MicrocapillaryRT-PCRRNA SequencingDNA MethylationFISHImmunostainingReporter Gene Assays

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Isolering og oprensning af kinesin fra Drosophila Embryoner

Histokemisk farvning af<em> Arabidopsis thaliana</em> Sekundært Cell vægelementer

Standardiseret metode til høj overførselshastighed Sterilisation af Arabidopsis frø

Billedbaserede metoder til at studere membranhandel i stomatale afstamningsceller

En højopløsning, enkeltkornet, in vivo pollenhydrering bioassay for Arabidopsis thaliana

Hairy Root Transformation and Regeneration in Arabidopsis thaliana and Brassica napus

Fremstilling af dobbeltfarvet F1 transgen arabidopsis thaliana

Read Article