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Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
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JoVE Journal Medicine
Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

DOI:
10.3791/50385-v

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11:27 min

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September 22, 2013

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, ,
1Department of Leukemia,MD Anderson Cancer Center, 2Wake Forest Baptist Medical Center,Institute for Regenerative Medicine

Chapters

  • 00:05Title
  • 02:06Syngeneic Murine Bone Marrow Transplantation (BMT)
  • 03:11Tumor Engraftment
  • 04:01Multi-parameter Immunofluorescent Staining
  • 06:13Multispectral Imaging
  • 07:10Quantitative Analysis of Multispectral Images
  • 08:00Results
  • 10:32Conclusion

Summary

Automatic Translation

Automatic Translation

Complex tissue masses, from organs to tumors, are composed of various cellular elements. We elucidated the contribution of cellular phenotypes within a tissue utilizing multi-labeled fluorescent transgenic mice in combination with multiparameter immunofluorescent staining followed by spectral unmixing to decipher cell origin as well as cell characteristics based on protein expression.

Tags

Keywords: Multispectral AnalysisCell OriginsCell TypesCell InteractionsTumor DevelopmentBone MarrowTumor StromaGFPRFPMIMiccMultiplexed Cellular CompositionsFluoroprobesImmunohistochemistryCell LineagesCell DifferentiationTumor Stromal Populations

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