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June 24, 2013
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We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.
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Cite this Article
Jun, S., Zhao, G., Ning, J., Gibson, G. A., Watkins, S. C., Zhang, P. Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions. J. Vis. Exp. (76), e50386, doi:10.3791/50386 (2013).
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