Journal
/
Bioengineering
/
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

DOI:
10.3791/50386-v

•

13:43 min

•

June 24, 2013

•

, , , , ,
1Department of Structural Biology,University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 02:53HeLa Cell Culture on Carbon-coated, Gold EM Finder Grids
  • 03:54HIV-1 Infection and Live-cell Imaging
  • 05:19Frozen-hydrated EM Sample Preparation
  • 06:14Cryo-fluorescence Light Microscopy
  • 07:51Cryo-electron Tomography
  • 08:56Three-dimensional Reconstruction using IMOD
  • 09:33Results: Observing Dynamic Behavior of Small HIV-1 Particles Interacting with Host HeLa Cells
  • 12:45Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.

Tags

Keywords: Correlative MicroscopyCryo-electron TomographyLive-cell ImagingHIV-1Virus-host Interactions3D Structural AnalysisFluorescence MicroscopyCryo-fluorescence Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50386?article_type=v

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
check_url/50386?article_type=v

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
check_url/50386?article_type=v

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
check_url/50386?article_type=v

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
check_url/50386?article_type=v

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
check_url/50386?article_type=v

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
check_url/50386?article_type=v

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System
check_url/50386?article_type=v

Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants
check_url/50386?article_type=v

3D Analysis of Multi-cellular Responses to Chemoattractant Gradients
check_url/50386?article_type=v

Four-Dimensional CT Analysis Using Sequential 3D-3D Registration

Read Article