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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

DOI:
10.3791/50415-v

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05:48 min

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May 23, 2013

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, , ,
1DFG-Center for Regenerative Therapies Dresden,Cluster of Excellence (CRTD) and Biotechnology Center (BIOTEC) of the Technische Universität Dresden

Chapters

  • 00:05Title
  • 01:23Preparation of the Injection Mixture and Apparatus
  • 02:49Anesthetization, Incision and Cerebroventricular Microinjection
  • 04:17Representative Cerebroventricular Morpholino Microinjection Results
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we demonstrate a method for manipulation of gene expression in the ventricular cells of the adult zebrafish telencephalon using antisense morpholino oligonucleotides. We present this method as an efficient and quick protocol that can be used for functional studies in the adult vertebrate brain.

Tags

Keywords: Gene ExpressionAdult Zebrafish BrainCerebroventricular MicroinjectionMorpholino OligonucleotidesRadial Glial CellsNeurogenesisNeurodegenerationCentral Nervous System Regeneration

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