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Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
JoVE Journal
Biology
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JoVE Journal Biology
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

DOI:
10.3791/50417-v

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08:00 min

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April 22, 2014

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1Molecular Neurobiology,Western University of Health Sciences, 2Graduate College of Biomedical Sciences,Western University of Health Sciences, 3College of Osteopathic Medicine of the Pacific,Western University of Health Sciences, 4College of Optometry,Western University of Health Sciences

Chapters

  • 00:05Title
  • 01:23Zebrafish Husbandry, Embryo Generation, and Treatment
  • 02:50Confocal Imaging of Cerebrovascular Structures in Fixed Zebrafish Embryos
  • 04:073D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 06:34Results: 3D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Imaging of cerebrovascular development in larval zebrafish is described. Techniques to facilitate 3D imaging and modify cerebrovascular development using chemical treatments are also provided.

Tags

Keywords: Zebrafish3D ReconstructionCerebrovascular StructuresEmbryonicImagingConfocal MicroscopyEGFPVascular Endothelial CellsBrainRetinaDevelopmental BiologyPathologyIn Vivo

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