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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
 

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Article doi: 10.3791/50436
July 13th, 2013 Usage Statistics

Summary July 13th, 2013

Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane proteins.

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