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Rekonstituering af et Kv Channel i lipidmembraner for strukturelle og funktionelle studier
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JoVE Journal Biology
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Rekonstituering af et Kv Channel i lipidmembraner for strukturelle og funktionelle studier

DOI:
10.3791/50436-v

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10:22 min

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July 13, 2013

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, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center at Dallas

Chapters

  • 00:05Title
  • 01:27Liposome Preparation and Detergent-induced Fusion of Vesicles
  • 04:15Removal of the Detergents to Form Proteoliposomes
  • 06:28Functional Study of the Ion Channel Activities in Black Lipid Membranes
  • 08:49Results: Two-Dimensional Crystallization of KvAP in Membranes
  • 09:56Conclusion

Summary

Automatic Translation

Automatic Translation

Procedurer for rekonstituering af en prototype spændingsstyret kaliumkanal i lipidmembraner beskrevet. De rekonstituerede kanaler er egnet til biokemiske analyser, elektriske optagelser, ligand screening og elektron krystallografiske studier. Disse metoder kan have generelle anvendelser til de strukturelle og funktionelle studier af andre membranproteiner.

Tags

Kv ChannelLipid ReconstitutionMembrane ProteinsVoltage-gated Potassium ChannelLipid-protein InteractionMembrane SystemsDetergent-induced Fusion2D Crystals

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