Journal
/
Neuroscience
/
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)

DOI:
10.3791/50446-v

•

10:57 min

•

July 25, 2013

•

, ,
1School of Life Sciences,Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

Chapters

  • 00:05Title
  • 02:40RNAi-mediated Double Gene Knockdown
  • 05:54Proboscis Extension Response (PER) Assay
  • 07:36Results: Double Kockdown of Vitellogenin (vg) and Ultraspiracle (usp) Genes affects Gustatory Perception
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

Tags

Keywords: RNAiDouble Gene KnockdownGustatory PerceptionProboscis Extension Response (PER) AssayHoney BeesApis MelliferaVitellogenin (vg)Ultraspiracle (usp)Gene ExpressionBehavioral MaturationForagingLearning And Memory

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50446?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/50446?article_type=v

Behavioural Pharmacology in Classical Conditioning of the Proboscis Extension Response in Honeybees (Apis mellifera)
check_url/50446?article_type=v

Creating Objects and Object Categories for Studying Perception and Perceptual Learning
check_url/50446?article_type=v

Using Affordable LED Arrays for Photo-Stimulation of Neurons
check_url/50446?article_type=v

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
check_url/50446?article_type=v

MPI CyberMotion Simulator: Implementation of a Novel Motion Simulator to Investigate Multisensory Path Integration in Three Dimensions
check_url/50446?article_type=v

Tactile Conditioning And Movement Analysis Of Antennal Sampling Strategies In Honey Bees (Apis mellifera L.)
check_url/50446?article_type=v

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
check_url/50446?article_type=v

A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees (Bombus terrestris)
check_url/50446?article_type=v

Neuropharmacological Manipulation of Restrained and Free-flying Honey Bees, Apis mellifera

Read Article