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Biology

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Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
 

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Article DOI: 10.3791/50448
June 25th, 2013

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Summary June 25th, 2013

The yeast mitochondrial nucleoid protein, Mgm101, is a Rad52-type recombination protein that forms large oligomeric rings. A protocol is described to prepare soluble recombinant Mgm101 using the Maltose Binding Protein (MBP)-tagging strategy coupled with cation exchange and size exclusion chromatography.

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