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Immunology and Infection
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4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

DOI:
10.3791/50450-v

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12:38 min

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August 13, 2013

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, , , ,
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology,Imperial College London, 2Preclinical Imaging,Caliper- A PerkinElmer Company

Chapters

  • 00:05Title
  • 01:21Preparing the Bacteria
  • 02:44Infection of Mice with Bioluminescent C. rodentium
  • 04:09Daily Composite 3D Diffuse Light Imaging Tomography with Integrated μCT Imaging (DLIT-μCT) of Infected Mice
  • 06:153D Reconstruction of DLIT-μCT Imaging Data
  • 09:29Generation of a 4D Movie of C. rodentium Infection
  • 10:24Monitored Infection with 5 x 109 cfu C. rodentium, Strain ICC180
  • 12:16Conclusion

Summary

Automatic Translation

Automatic Translation

Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.

Tags

Keywords: 4D Multimodality ImagingCitrobacter RodentiumBioluminescenceDLIT-μCTLongitudinal MonitoringBacterial InfectionColitisMouse ModelIVIS Spectrum CT3D Optical-CT Co-registrationLow-dose μCTBacterial Colonization Dynamics

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