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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
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4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

Article DOI: 10.3791/50450
August 13th, 2013 •
1, 2, 2, 2, 1
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology, Imperial College London, 2Preclinical Imaging, Caliper- A PerkinElmer Company

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August 13th, 2013

Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.

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