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Anskaffelse Fluorescens Time-lapse filmer av Spirende gjær og analysere encellede Dynamics bruke grafts
JoVE Journal
Biology
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JoVE Journal Biology
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
DOI:

17:01 min

July 18, 2013

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Chapters

  • 00:05Title
  • 01:36Obtain Fluorescent Microscopy Movies of Single Yeast Cells Growing in a Microfluidic Chamber
  • 06:33Format and Segment Data for Tracking Using the FormatData GUI in MATLAB
  • 09:00Track Cells and Lineages through Time with ProcessTimeSeries GUI, and Curate ID and Lineage Assignments
  • 12:33Exporting Data for Analysis
  • 13:35Results: Analysis of Single-cell Time Series
  • 16:22Conclusion

Summary

Automatic Translation

Vi presenterer en enkel protokoll for å få fluorescens mikroskopi filmer av voksende gjærceller, og et GUI-basert programvare pakke for å trekke encellede tidsserier. Analysen omfatter automatiserte avstamning og divisjon tid oppdraget integrert med visuell inspeksjon og manuell curation sporede data.

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