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Chapters
- 00:05Title
- 01:36Obtain Fluorescent Microscopy Movies of Single Yeast Cells Growing in a Microfluidic Chamber
- 06:33Format and Segment Data for Tracking Using the FormatData GUI in MATLAB
- 09:00Track Cells and Lineages through Time with ProcessTimeSeries GUI, and Curate ID and Lineage Assignments
- 12:33Exporting Data for Analysis
- 13:35Results: Analysis of Single-cell Time Series
- 16:22Conclusion
Vi presenterer en enkel protokoll for å få fluorescens mikroskopi filmer av voksende gjærceller, og et GUI-basert programvare pakke for å trekke encellede tidsserier. Analysen omfatter automatiserte avstamning og divisjon tid oppdraget integrert med visuell inspeksjon og manuell curation sporede data.
