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Chapters
- 00:05Title
- 01:01Preparing Antibody-conjugated Beads and Collagen Plates
- 02:18Excising and Digesting Heart
- 04:05Isolating Endothelial Cells
- 06:21Representative Endothelial Cell Isolation Results
- 07:36Conclusion
Primary cell culture is a useful technique for analyzing specific populations of cells, particularly from transgenic mouse embryos at specific developmental stages. Herein, embryonic ventricles are dissected and dissociated, and antibody-conjugated beads recognize and separate out the endothelial cells for further analysis.
