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Biology
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糖原生化滴定<em>在体外</em
JoVE Journal
Biology
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JoVE Journal
Biology
Biochemical Titration of Glycogen
In vitro
Please note that all translations are automatically generated.
Click here for the English version.
糖原生化滴定<em>在体外</em
DOI:
10.3791/50465-v
•
07:16 min
•
November 24, 2013
•
Joffrey Pelletier
,
Grégory Bellot
,
Jacques Pouysségur
,
Nathalie M. Mazure
1
Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 – INSERM, U1081 – UNS, Centre Antoine Lacassagne
,
University of Nice – Sophia Antipolis
Chapters
00:05
Title
00:54
Cell Lysis
02:50
Hydrolysis of Glycogen
03:55
Development and Reading of Fluorescence
05:28
Results: Glycogen Quantification and Its Role in Hypoxia
06:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述的糖原
体外
滴定准确,重现性好,方便的生化方法。这种技术使用了Abcam公司糖原测定试剂盒和基于糖原的连续水解的荧光成葡萄糖和葡萄糖滴定。
Tags
Glycogen
Biochemical Titration
In Vitro
Glucose
Glucoamylase
OxiRed Probe
Fluorescence
Cell Line
CCL39
LS174
Normoxia
Hypoxia
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