Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive <em>In vivo</em> Imaging with Single-cell Resolution in Real-time

Midhat H. Abdulreda; Alejandro Caicedo; Per-Olof Berggren

doi:10.3791/50466

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Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

JoVE Journal
Medicine
Author Produced

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JoVE Journal Medicine

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

13,323 Views

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05:54 min

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March 10, 2013

DOI:

10.3791/50466-v

DOI:

10.3791/50466-v

•

05:54 min

March 10, 2013

•

16 Views

Midhat H. Abdulreda^1,2, Alejandro Caicedo^1,3,4, Per-Olof Berggren^1,5

¹Diabetes Research Institute,University of Miami Miller School of Medicine, ²Department of Surgery,University of Miami Miller School of Medicine, ³Department of Medicine,University of Miami Miller School of Medicine, ⁴Department of Physiology & Biophysics,University of Miami Miller School of Medicine, ⁵The Rolf Luft Research Center for Diabetes and Endocrinology,Karolinska Institutet

Summary

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.