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Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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JoVE Journal Medicine
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

DOI:
10.3791/50466-v

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05:54 min

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March 10, 2013

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, ,
1Diabetes Research Institute,University of Miami Miller School of Medicine, 2Department of Surgery,University of Miami Miller School of Medicine, 3Department of Medicine,University of Miami Miller School of Medicine, 4Department of Physiology & Biophysics,University of Miami Miller School of Medicine, 5The Rolf Luft Research Center for Diabetes and Endocrinology,Karolinska Institutet

Chapters

  • 00:01Title
  • 00:43Motorized-syringe driver with syringe assembled
  • 00:56Loading islets into cannula
  • 03:13Islet Transplantation
  • 05:00Representative Results

Summary

Automatic Translation

Automatic Translation

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.

Tags

Keywords: Intravital ImagingNon-invasive ImagingLongitudinal ImagingSingle-cell ResolutionAnterior Chamber Of The EyeTransplantationPancreatic IsletIn VivoDrug ScreeningSignal TransductionCancerAutoimmune Disease

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