Anterior Chamber Transplantation
Anterior Chamber Transplantation - Related Videos
05:54
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
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Cited by 31 •
2013
A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
05:27
In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye
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2024
Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.
09:59
An “All-laser” Endothelial Transplant
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Cited by 5 •
2015
An all laser procedure is proposed in the endothelial transplant. The surgical technique is based on the use of a femtosecond laser to prepare the donor tissue. Laser welding technique is then used to secure the donor endothelium in the correct position.
FAQs
The iris provides a surface where the graft can remain positioned within the aqueous-filled anterior chamber and establish local vascular connections. Because the graft is not hidden behind opaque tissue, researchers can repeatedly examine its behavior through the transparent cornea. This arrangement connects the biological events of transplantation with direct in vivo imaging over time.
Anterior chamber transplantation can support longitudinal assessment of islet survival, vascularization, inflammation, and immune-mediated rejection. Repeated observation allows these processes to be examined as changing biological events rather than as findings from a single endpoint. This is particularly useful for relating graft condition to functional responses and to the effects of experimental treatments.
Longitudinal intravital microscopy enables repeated, minimally invasive imaging of the same graft in vivo. Researchers can therefore follow graft behavior and functional changes over time while assessing vascular connections, inflammatory responses, or rejection-related changes. The approach provides a visual and functional record that strengthens studies of how transplanted tissue responds within the host.
After placement on the iris, investigators can assess how the graft survives, develops local vascular connections, and responds to the surrounding environment. Imaging through the cornea supports direct evaluation, while functional assessment adds information beyond appearance alone. For pancreatic islets, these observations help characterize graft biology in an experimental transplantation setting.
Researchers use this experimental platform when they need to investigate pancreatic islet graft biology in vivo, including survival, vascularization, inflammation, and immune-mediated rejection. Its visibility supports repeated assessment during diabetes-related studies, making it useful for examining how islet grafts behave and how their condition changes during experimental interventions.
The model links transplanted tissue with direct, repeated observation and functional assessment, allowing investigators to examine therapeutic responses in living subjects. In diabetes research, this can reveal whether a cell-based treatment is associated with changes in islet graft behavior, vascularization, survival, inflammation, or rejection. Its minimally invasive design supports monitoring during the treatment study.