/
/
Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:34Retinal Preparation
- 02:22Water Washes and Trypsin Digest
- 03:33Separating the Vasculature
- 05:32Flat-mounting the Vasculature
- 06:13Results: Representative Images of Trypsin Digest
- 08:07Conclusion
Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.
