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JoVE Journal
Neuroscience

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Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model
 

Trypsin Digest Protocol to Analyze the Retinal Vasculature of a Mouse Model

Article DOI: 10.3791/50489
June 13th, 2013

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Summary June 13th, 2013

Trypsin digest is one of the most commonly used methods to analyze retinal vasculature. This manuscript describes the method in detail, including key alterations to optimize the technique and remove the non-vascular tissue while preserving the overall architecture of the vessels.

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