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Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy
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Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

Visualization of Craniofacial Development in the sox10: kaede Transgenic Zebrafish Line Using Time-lapse Confocal Microscopy

DOI:
10.3791/50525-v

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06:35 min

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September 30, 2013

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, ,
1Center for Regenerative Medicine,Massachusetts General Hospital

Chapters

  • 00:05Title
  • 01:28Mounting Embryos
  • 02:21Photoconversion Using Confocal Microscopy
  • 05:04Representative Photoconversion Results
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

Visualization of experimental data has become a key element in presenting results to the scientific community. Generation of live time-lapse recording of growing embryos contributes to better presentation and understanding of complex developmental processes. This protocol is a step-by-step guide to cell labeling via photoconversion of kaede protein in zebrafish.

Tags

Keywords: Craniofacial DevelopmentSox10: Kaede Transgenic ZebrafishTime-lapse Confocal MicroscopyVertebrate PalatogenesisCranial Neural Crest CellsLineage AnalysisMaxillaryMandibularEthmoid PlatePhotoconvertible Reporter ProteinZebrafish Embryo

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