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Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae
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Biology
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Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

DOI:
10.3791/50539-v

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08:25 min

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July 23, 2013

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, , ,
1Department of Biomedical Engineering,University of Michigan , 2Department of Surgery, Section of Plastic Surgery,University of Michigan , 3Departments of Pediatrics and Neurology,University of Michigan , 4Department of Molecular and Integrative Physiology,University of Michigan

Chapters

  • 00:05Title
  • 01:12Preparing Suture Loops
  • 01:50Preparing the Experimental Chamber and Positioning the Larva
  • 04:11Recording Force During a Maximal Twitch Contraction
  • 06:20Measuring Musculature Dimensions with Larva at Optimal Length
  • 07:00Results: Peak Force During Maximal Twitch
  • 07:56Conclusion

Summary

Automatic Translation

Automatic Translation

Force measurements can be used to demonstrate changes in muscle function due to development, injury, disease, treatment or chemical toxicity. In this video, we demonstrate a method to measure force during a maximal contraction of zebrafish larval trunk muscle.

Tags

Keywords: Force MeasurementMuscle FunctionZebrafish LarvaeMuscle ContractionIsometric TwitchForce TransducerLength ControllerElectric Field StimulationMuscle DevelopmentMuscle DiseaseMuscle Toxicity

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