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Behavior
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立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱
JoVE Journal
Behavior
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Behavior
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
Please note that all translations are automatically generated.
Click here for the English version.
立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱
DOI:
10.3791/50600-v
•
08:22 min
•
July 30, 2013
•
Kathryn C. Schierberl
2
,
Anjali M. Rajadhyaksha
2
1
Graduate Program in Neuroscience
,
Weill Cornell Graduate School of Biomedical Sciences
,
2
Division of Pediatric Neurology, Department of Pediatrics
,
Weill Cornell Medical College
Chapters
00:05
Title
01:36
Preparation and Setup for Stereotaxic Delivery of Viral Vectors
02:32
Stereotaxic Microinjection of Viral Vectors
04:41
Conditioned Place Reference: Acquisition, Extinction, and Reinstatement
05:55
Representative Cocaine Conditioned Place Preference Results
07:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍如何microinject病毒载体到小鼠大脑中,然后在条件性位置偏爱的范例,包括收购,灭绝和恢复阶段测试。
Tags
Stereotaxic Microinjection
Viral Vectors
Cre Recombinase
Conditional Gene Knockout
Cocaine
Conditioned Place Preference
Addiction
Behavioral Models
Temporally-specific
Regionally-specific
Gene Deletion
Gene Targeting
RAAV
Cocaine-induced Plasticity
Withdrawal
Extinction
Reinstatement
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