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立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱

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JoVE Journal Behavior

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

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立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱

DOI:

10.3791/50600-v

•

08:22 min

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July 30, 2013

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Kathryn C. Schierberl², Anjali M. Rajadhyaksha²

¹Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, ²Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Chapters

00:05Title
01:36Preparation and Setup for Stereotaxic Delivery of Viral Vectors
02:32Stereotaxic Microinjection of Viral Vectors
04:41Conditioned Place Reference: Acquisition, Extinction, and Reinstatement
05:55Representative Cocaine Conditioned Place Preference Results
07:39Conclusion

Summary

Automatic Translation

本文介绍如何microinject病毒载体到小鼠大脑中，然后在条件性位置偏爱的范例，包括收购，灭绝和恢复阶段测试。

Tags

Keywords: Stereotaxic Microinjection Viral Vectors Cre Recombinase Conditional Gene Knockout Cocaine Conditioned Place Preference Addiction Behavioral Models Temporally-specific Regionally-specific Gene Deletion Gene Targeting RAAV Cocaine-induced Plasticity Withdrawal Extinction Reinstatement

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