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JoVE Journal
Bioengineering

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Permeabilization of Adhered Cells Using an Inert Gas Jet
 

Permeabilization of Adhered Cells Using an Inert Gas Jet

Article DOI: 10.3791/50612 08:21 min
September 4th, 2013

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Summary September 4th, 2013

This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.

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