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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

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Biology

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Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

Separation of Spermatogenic Cell Types Using STA-PUT Velocity Sedimentation

DOI:

10.3791/50648-v

•

09:48 min

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October 09, 2013

•

Jessica M Bryant², Mirella L Meyer-Ficca, Vanessa M Dang, Shelley L Berger^4,5, Ralph G Meyer

¹Department of Cell and Developmental Biology,University of Pennsylvania, ²Biomedical Graduate Studies,University of Pennsylvania, ³Department of Animal Biology and Center for Animal Transgenesis and Germ Cell Research,University of Pennsylvania, ⁴Department of Genetics,University of Pennsylvania, ⁵Department of Biology,University of Pennsylvania

Chapters

00:05Title
01:07STA-PUT Apparatus
03:50Isolating Spermatogenic Cells from Whole Testes
05:28Cell Loading and Sedimentation
08:04Results: STA-PUT Purification of Testes
09:20Conclusion

Summary

Automatic Translation

The STA-PUT method allows for the separation of different populations of spermatogenic cells based on size and density.

Tags

Keywords: Spermatogenic Cell Types STA-PUT Velocity Sedimentation Spermatogenesis Testis Cell Types Cell Isolation Cell Enrichment BSA Gradient Sedimentation Velocity Cell Yield FACS Elutriator

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