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在大规模的基因敲除

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

Journal (Biology)

Induction of Right Ventricular Failure by Pulmonary Artery Constriction and Evaluation of Right Ventricular Function in Mice

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Journal (Biology)

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

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在大规模的基因敲除

Article DOI: 10.3791/50693 • 18:38 min

September 25th, 2013 •

Kathryn N. Maher¹, Mary Catanese², Daniel L. Chase³

¹Molecular and Cellular Biology Program, University of Massachusetts, Amherst, ²Neuroscience and Behavior Program, University of Massachusetts, Amherst, ³Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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September 25th, 2013

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而喂养的dsRNA在C线虫是一种功能强大的工具来评估基因功能，为大规模饲养屏幕当前的协议导致变量击倒效率。我们描述了一种改进的协议用于进行大规模的RNAi馈送屏幕，导致基因表达的高度有效的和可再现的击倒。

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