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脂肪组织免疫细胞的分离
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Adipose Tissue Immune Cells
DOI:

07:09 min

May 22, 2013

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Chapters

  • 00:05Title
  • 01:11Harvesting and Preparation of Adipose Tissue
  • 02:06Collagenase Digestion
  • 03:19Staining of Cell Surface Antigens
  • 04:23FACS Analysis
  • 05:13Representative Results
  • 06:54Conclusion

Summary

Automatic Translation

脂肪组织(AT)是一个强烈的免疫细胞的激活和相互作用的部位。几乎所有的免疫系统的细胞是存在于AT和它们的比率是改变了肥胖。适当的隔离,定量,定性的AT免疫细胞群,是了解自己的角色在immunometabolic疾病的关键。

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